General Provisions

1. The Molecular Laboratory is a part of the Institute of Environmental Sciences at the Jagiellonian University. It includes laboratories 2.2.9, 2.2.10, and 2.2.11.
2. The head of the laboratory is Prof. Dr. Hab. Wiesław Babik from the Genomics and Experimental Evolution Group. For administrative matters, contact Dr. Katarzyna Dudek (room 2.2.5, internal phone 6870).
3. Only individuals authorized by the head of the laboratory are permitted in the Laboratory.
4. Students may use the Laboratory only with the consent of the laboratory head. To obtain permission, the following conditions must be met:

• Present written consent from the supervisor of the bachelor's or master's thesis to the laboratory head.
• Complete training on the laboratory's operation and pipetting procedures.
• Familiarize oneself with this Regulation and confirm it in writing.

5. The supervisor must grant permission for the student to use the laboratory and commit to supervising the student's work.
6. Prior to commencing work on a new project, notification must be provided to the laboratory head. Information about project participants and the techniques to be used must be submitted.
7. Failure to comply with this regulation may result in the withdrawal of permission to use the laboratory. Violations by students may have consequences for their supervisors.

Laboratory Organization

1. Room 2.2.9 is POST-PCR laboratory, while laboratories 2.2.10 and 2.2.11 are used for PRE-PCR work.
2. It is prohibited to transfer any objects (pipettes, glassware, plastics) or reagents between laboratories.
3. All reagents, buffers, and plastic consumables (eppendorf tubes, pipette tips) must be labeled with the name of the department. Using items from another department is not allowed.
4. The Laboratory contains items for general use (reagents, stands, tips) labeled "PM" or "LEM" (without the department's name).
5. Each department receives a labeled shelf in the refrigerator and freezer. Samples must not be placed on shelves belonging to other departments. Samples must be labeled; otherwise, they will be removed.

Safety

1. All activities must be conducted in accordance with health and safety regulations and specific instructions.
2. Those handling hazardous substances must possess the safety data sheet for the substance, familiarize themselves with its contents, and apply the prescribed safety measures.
3. Work with biological material (DNA, RNA) should be conducted while wearing gloves.
4. If working conditions do not comply with health and safety regulations and pose an immediate risk to health or life, work should be suspended, and superiors should be notified promptly.
5. After completing work, the following steps must be taken:

• Clean the work area, paying particular attention to the proper storage of hazardous substances and chemicals.
• Disinfect the table with a chlorine solution.
• Ensure that equipment and machinery are adequately secured to prevent accidents, such as falls or accidental gas leaks.
• Turn off the electrical supply to machines and equipment, check the closure of water and gas installations. If equipment is intentionally left on, leave information about the responsible person.

6. All items left out of place after work will be removed.

Miscellaneous Notes

1. There is a first-aid kit in the laboratory equipped with necessary pharmaceuticals and medical materials.
2. Maintenance and repair work on electrical installations and equipment may only be carried out by individuals with appropriate qualifications. Any equipment or installation failures should be reported immediately to the department head or technical services (phone: 6744, 5135, 5121).
3. Consuming meals and beverages in the laboratories is prohibited.
4. Entering the laboratories with coats, jackets, bags, and backpacks is not allowed.
5. Laboratory windows may only be opened in emergency situations.